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Updated: Jun 18, 2026
13:48
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Noninvasive molecular imaging of small living subjects using Raman spectroscopy
1Molecular Imaging Program at Stanford, Departments of Radiology and Bioengineering, Bio-X Program, Stanford University, 1201 Welch Road, Stanford, CA 94305-5484, USA.
Summary
Researchers developed a novel deep-tissue molecular imaging technique using Raman spectroscopy and specialized nanoparticles. This noninvasive method enables whole-body imaging in small animals, showing potential for advanced biomedical applications.
Area of Science:
- Biomedical Imaging
- Molecular Imaging
- Spectroscopy
Background:
- Bioluminescence and fluorescence imaging are common for small-animal models.
- Noninvasive deep-tissue molecular imaging requires advanced techniques.
Purpose of the Study:
- To present a novel noninvasive deep-tissue molecular imaging strategy using Raman spectroscopy.
- To demonstrate the capability of Raman spectroscopy and nanoparticles for small-animal imaging.
Main Methods:
- Utilized surface-enhanced Raman scattering (SERS) nanoparticles and single-wall carbon nanotubes.
- Developed a small-animal Raman imaging system for whole-body imaging.
- Investigated nanoparticle pharmacokinetics and in vivo tumor targeting.
Main Results:
- Achieved whole-body Raman imaging in living subjects.
- Demonstrated successful nanoparticle pharmacokinetics tracking.
- Showcased multiplexing capabilities and in vivo tumor targeting with Raman nanoparticles.
Conclusions:
- Raman spectroscopy offers a promising noninvasive modality for deep-tissue molecular imaging.
- The developed strategy holds significant potential for biomedical imaging applications in living subjects.
Related Concept Videos
01:20Raman Spectroscopy: Overview
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
01:26Raman Spectroscopy Instrumentation: Overview
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...