Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Recent progress in membrane dynamics research by two-photon microscopy].

Tomomi Nemoto1

  • 1National Institutes of Natural Sciences, National Institute for Physiological Sciences, Okazaki City, Japan. tn@nips.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|April 2, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Localized heat induces ERK activation and signal propagation in solid tumors.

Scientific reports·2026
Same author

Ether phospholipids modulate somatosensory responses by tuning multiple receptor functions in <i>Drosophila</i>.

iScience·2026
Same author

Planar cell polarity emerges through polarized accumulation of Wnt11.

Science advances·2026
Same author

Construction of Large Cranial Windows With Nanosheet and Light-Curable Resin for Long-term Two-Photon Imaging in Mice.

Bio-protocol·2025
Same author

Body temperature regulates glucose metabolism and torpid behavior.

Nature communications·2025
Same author

Absence of the axon initial segment in sensory neuron enhances resistance to amyotrophic lateral sclerosis.

Brain : a journal of neurology·2025
Same journal

[Effect of Application Device Design on Scalp Delivery of Topical Minoxidil Formulations].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Survey of Pharmaceutical Interventions in Outpatient Cancer Chemotherapy].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

Effects of Fluctuating Triglyceride Levels on Initial and Steady-state Serum Amiodarone Concentrations in Japanese Patients: A Retrospective Study.

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Dynamic Oligomerization Processes and Functional Characterization of Bacterial ClpP Protease].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Structural Analysis Using Cryo-electron Microscopy for Protein-based Drug Design].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
Same journal

[Expansion of Targeted Protein Degradation for Targeting Aggregate-prone Proteins and Mitochondrial Proteins].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2026
See all related articles

Two-photon microscopy enables long-term visualization of deep living cells and reveals "sequential compound exocytosis" in various cell types. This advanced imaging technique also visualizes neural structures in vivo.

Area of Science:

  • * Advanced bioimaging techniques
  • * Cellular and molecular biology
  • * Neuroscience

Context:

  • * Two-photon microscopy offers less-invasive, cross-sectional imaging for deep tissue visualization.
  • * It utilizes multi-photon excitation for long-term observation of living cells.
  • * Widely applied in biological and medical sciences for cellular processes.

Purpose:

  • * To demonstrate
  • sequential compound exocytosis
  • in pancreatic acinar cells using two-photon microscopy.
  • * To explore the dynamics of actin cytoskeleton and SNARE proteins in exocytosis.
  • * To develop and apply novel methods like TEPIQ for high-resolution vesicle analysis.

Summary:

  • * Two-photon microscopy successfully visualized deep neural structures in vivo, including neurons and glial cells.

Related Experiment Videos

  • * The study identified and characterized
  • sequential compound exocytosis
  • in diverse secretory cells.
  • * Novel methods were developed to determine fusion pore characteristics and vesicle size beyond diffraction limits.

    • Impact:

    • * Established
    • sequential compound exocytosis
    • as a fundamental process in various cell types.
    • * Enabled in vivo visualization of neural and glial cell dynamics in living mice.
    • * Advanced the study of molecular mechanisms underlying physiological and pathological events.