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Related Concept Videos

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

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Related Experiment Video

Updated: Jul 6, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

ChIPping away at gene regulation.

Charles E Massie1, Ian G Mills

  • 1Uro-Oncology Research Group, CRUK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK.

EMBO Reports
|April 2, 2008
PubMed
Summary

Understanding gene expression regulation in eukaryotes is challenging. New technologies offer genome-wide insights into transcription factor networks, but further advancements are needed for a complete picture.

Area of Science:

  • Molecular Biology
  • Genomics
  • Systems Biology

Background:

  • Coordinated gene expression regulation in higher eukaryotes is intricate and not fully understood.
  • Genome-wide studies are beginning to reveal insights into gene regulatory networks.
  • Transcription factor interactions are key to understanding gene expression.

Purpose of the Study:

  • To review current technological advances in studying gene expression regulation.
  • To identify knowledge gaps in understanding complex gene regulatory pathways.
  • To emphasize the need for novel technologies in this field.

Main Methods:

  • Genome-wide analyses of transcription factor binding sites.
  • Computational prediction of cooperative interactions between transcription factors.

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CRISPR-Mediated Reorganization of Chromatin Loop Structure
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CRISPR-Mediated Reorganization of Chromatin Loop Structure

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Last Updated: Jul 6, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

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CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

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  • Review of existing methodologies and technological limitations.

    • Main Results:

    • Current methods have provided initial insights into transcription factor target sites and interactions.
    • Genome-wide approaches have begun to map gene regulatory networks.
    • Significant gaps remain in comprehensively understanding the coordination of gene expression.

    Conclusions:

    • Technological progress has advanced the study of gene expression regulation.
    • New technologies are essential to overcome current limitations and fully map complex regulatory pathways.
    • Further research is required to elucidate the complete mechanisms of coordinated gene expression.