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Updated: Jul 6, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
[Chemical characteristic and serological activity of Pragia fontium lipopolysaccharides]
Abstract:
Lipopolisaccharides (LPS) of 8 strains of Pragia fontium--representatives of a new genus of Enterobacteriaceae family, was isolated for the first time. It was demonstrated, that lipopolysaccharides yields were 10-20% of dry cells weight. Investigations of monosaccharide composition have determined the presence of glucose, galactose, rhamnose and mannose in lipopolysaccharides, that permitted to establish 4 chemotypes ofpolysaccharide part of LPS. Serological investigations showed immunochemical heterogeneity of Pfontium: 8 investigated strains were divided by the O-antigenicity into 7 serogroups.

