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Related Experiment Videos

Cholesterol esterase activity in body fluids.

K C Watson, E J Kerr

    British Journal of Experimental Pathology
    |August 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Elevated antistreptolysin O activity in bodily fluids is often due to cholesterol in lipoproteins, not antibodies. Cholesterol esterase in these fluids also impacts activity levels.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • Antistreptolysin O (ASO) is a common diagnostic marker.
    • Elevated ASO titers typically indicate recent streptococcal infection.
    • The source of ASO activity in certain effusions was investigated.

    Purpose of the Study:

    • To investigate the nature of antistreptolysin O activity in ascitic, pleural, and joint fluids.
    • To determine if the observed activity is antibody-mediated or due to other serum components.
    • To explore the role of cholesterol esterase in these fluids.

    Main Methods:

    • Analysis of antistreptolysin O activity in patient effusions.
    • Precipitation assays using dextran sulphate.
    • Incubation experiments with patient fluids and normal human serum.

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  • Assessment of cholesterol esterase activity.

    • Main Results:

    • Significant antistreptolysin O activity (≥200 Todd units/ml) detected in 20% of ascitic, 20% of pleural, and 37.5% of joint fluids.
    • This activity was attributed to altered beta-lipoproteins, not antibodies.
    • Cholesterol esterase activity was present in 90% of ascitic, 59% of pleural, and 54% of joint fluids, influencing ASO titers upon serum incubation.

    Conclusions:

    • Antistreptolysin O activity in effusions can originate from lipoprotein cholesterol, not solely from antibodies.
    • Cholesterol esterase plays a significant role in modulating ASO activity in these fluids.
    • Further investigation is needed to understand esterase activity and lipoprotein interactions in effusions.