Related Experiment Video

Updated: Jul 6, 2026

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
09:43

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Published on: March 8, 2024

[Effect of fibrinogen on the secretion of interleukin-1beta and - 8 by polymorphonuclear leukocytes]

Song Ge1, Ya-fei Wu, Tian-jia Liu

  • 1Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology
|April 3, 2008
PubMed
Abstract

Insights

Fibrinogen, an acute-phase protein, significantly increases the release of inflammatory cytokines IL-1beta and IL-8 from polymorphonuclear leukocytes (PMN). This suggests fibrinogen may amplify inflammatory responses in periodontium, potentially contributing to bone resorption.

Area of Science:

  • Immunology
  • Biochemistry
  • Periodontology

Background:

  • Acute-phase proteins play a role in inflammation.
  • Fibrinogen is an acute-phase protein.
  • Polymorphonuclear leukocytes (PMN) are key inflammatory cells.

Purpose of the Study:

  • To investigate the effect of fibrinogen on IL-1beta and IL-8 release from human PMN.
  • To explore the role of fibrinogen in periodontium destruction.

Main Methods:

  • Human peripheral PMN were isolated using density gradient centrifugation.
  • PMN were cultured and stimulated with varying concentrations of human fibrinogen.
  • Interleukin (IL)-1beta and IL-8 levels in supernatants were quantified using ELISA.

Main Results:

  • Fibrinogen stimulation significantly increased IL-1beta and IL-8 release from PMN compared to controls.
  • Higher fibrinogen concentrations and longer incubation times resulted in greater cytokine release.
  • The increase in IL-1beta and IL-8 was statistically significant (P < 0.001 for IL-1beta, P <= 0.016 for IL-8).

Conclusions:

  • Fibrinogen induces the secretion of pro-inflammatory cytokines IL-1beta and IL-8 by PMN.
  • Fibrinogen may exacerbate inflammatory responses in the periodontium.
  • Fibrinogen's role in periodontium inflammation and bone resorption warrants further investigation.

More Related Videos

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1&beta; using a Whole Blood Stimulation Assay06:29

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Related Concept Videos

Acute Inflammation III: Local and Systemic Effects01:25

Acute Inflammation III: Local and Systemic Effects

Acute inflammation produces a coordinated set of local and systemic changes that limit injury, eliminate pathogens, and initiate repair. These responses arise within minutes of infection, trauma, or chemical insult and are driven by vascular alterations and leukocyte-derived mediators. When the stimulus resolves, the reaction typically abates within days.Local EffectsAt the site of injury, arteriolar vasodilation increases blood flow, resulting in redness and warmth. Simultaneously, increased...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Inflammation01:38

Inflammation

Overview