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Updated: Jul 6, 2026
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gut microbiota and its possible relationship with obesity
John K DiBaise1, Husen Zhang, Michael D Crowell
1Division of Gastroenterology and Hepatology, Mayo Clinic, 13400 E Shea Blvd, Scottsdale, AZ 85259, USA. dibaise.john@mayo.edu
Mayo Clinic Proceedings
|April 3, 2008
Summary
The gut microbiota influences nutrient absorption and energy storage, potentially contributing to obesity. Modifying gut bacteria may offer a future treatment strategy for obesity and weight management.
Area of Science:
- Microbial ecology
- Human physiology
- Metabolic syndrome
Background:
- Obesity arises from dysregulated energy balance.
- The gut microbiota's role in nutrient acquisition and energy regulation is increasingly recognized.
- Microbiota composition differs between lean and obese individuals.
Purpose of the Study:
- To review evidence linking gut microbiota to obesity development.
- To explore the therapeutic potential of microbiota modulation for obesity treatment.
Main Methods:
- Review of published literature on gut microbiota and obesity.
- Analysis of studies in animal models and human subjects.
- Examination of microbial composition differences (e.g., Bacteroidetes, Firmicutes).
Main Results:
- Gut microbiota aids in calorie extraction and storage in adipose tissue.
- Obese individuals often exhibit a higher Firmicutes to Bacteroidetes ratio.
- Bacterial lipopolysaccharide may link gut inflammation to metabolic syndrome.
Conclusions:
- Gut microbial ecology plays a significant role in host energy homeostasis.
- Targeting gut microbiota modification presents a promising avenue for obesity treatment.
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