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Updated: Jul 6, 2026

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
Published on: December 19, 2020
Synthesis and in vivo studies of carbohydrate-based vaccines against group A streptococcus
Pavla Simerska1, Abu-Baker M Abdel-Aal, Yoshio Fujita
1School of Molecular and Microbial Sciences (SMMS), The University of Queensland, St Lucia, Queensland 4072, Australia. p.simerska@uq.edu.au
Abstract:
Carbohydrates, as carriers, providing numerous attachment points for the conjugation of peptide antigens and their optimal orientation for the recognition by cells of the immune system, reducing degradation of the attached peptide antigens and many other advantages make carbohydrate-based vaccine highly promising approach. Multiple copies of a single group A streptococcal (GAS) M protein derived specific peptide antigens (J8 or J14) were coupled onto carbohydrate cores (D-glucose and D-galactose) linked to lipophilic amino acids to produce a self-adjuvanting liposaccharide vaccine against GAS strains. In vivo experiments showed high serum IgG antibody titers against each of the incorporated peptide epitopes, J8 or J14.
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