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Updated: Jul 6, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
Bioactive peptides derived from the Limulus anti-lipopolysaccharide factor: structure-activity relationships and
Puig Mora1, Manuela López De La Paz, Enrique Pérez-Payá
1Department of Medicinal Chemistry. Centro de Investigación Príncipe Felipe. Avda Autopista del Saler, 16. E-46013 Valencia, Spain.
Abstract:
The design of peptides that would interact and neutralise bacterial endotoxins or LPS could have benefited from the analysis of comparative structure-activity relationships among close-related analogues. Here, we present a comparative structural characterisation of selected peptides derived from the LALF obtained by single-amino-acid replacement, which differ in biological activity. The peptides were characterised in solution using nuclear magnetic resonance, circular dichroism and fluorescence spectroscopies. Membrane mimetic peptide interactions were studied using fluorescence resonance energy transfer with the aid of extrinsic fluorescent probes that allowed the identification of mixed peptide/lipid complexes.
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