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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jul 6, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

Unlocking pathology archives for microRNA-profiling.

Kai P Hoefig¹, Christoph Thorns, Anja Roehle

¹Institute for Pathology, UKSH Campus Luebeck, Luebeck, Germany.

Anticancer Research

|April 4, 2008

Summary

This summary is machine-generated.

Formalin-fixed, paraffin-embedded (FFPE) tissue is suitable for microRNA (miRNA) profiling. FFPE processing minimally impacts miRNA expression profiles, making it reliable for cancer research.

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Area of Science:

Molecular Biology
Genomics
Cancer Research

Background:

MicroRNAs (miRNAs) are key regulators of gene expression.
Dysregulated miRNA expression is implicated in numerous human cancers.
Formalin-fixed, paraffin-embedded (FFPE) tissues are standard for retrospective studies.

Purpose of the Study:

To evaluate the suitability of FFPE tissues for miRNA profiling.
To compare miRNA expression profiles between fresh-frozen (CRYO) and FFPE tissues.

Main Methods:

Quantitative PCR (qPCR)-based assay.
Profiling of 157 miRNA species.
Comparison of miRNA profiles from seven tonsil and four liver samples (CRYO vs. FFPE).

Main Results:

FFPE processing had minimal impact on miRNA significance.
Variance introduced by FFPE treatment was less than biological variation.
MiRNA profiles from FFPE tissues were comparable to CRYO tissues.

Conclusions:

FFPE tissues are well-suited for miRNA profiling.
FFPE is a reliable material for retrospective miRNA studies in cancer research.