Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
Drug Concentrations: Measurements01:23

Drug Concentrations: Measurements

Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
Plasma —...
Toxicity Testing in Animals01:23

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Serum Laboratory Studies, Stool Test, Breath Test01:30

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal 3D-printed passive samplers to monitor, model and prioritise <i>in situ</i> pharmaceutical and pesticide pollution risks to an aquatic freshwater invertebrate, <i>Gammarus pulex</i>.

Environmental science. Processes & impacts·2025
Same author

A Novel High-Throughput Immunoaffinity LC-MS/MS Assay for P-III-NP and Other Fragments of Type III Procollagen in Human Serum.

Drug testing and analysis·2024
Same author

TREM2<sup>+</sup> and interstitial-like macrophages orchestrate airway inflammation in SARS-CoV-2 infection in rhesus macaques.

Nature communications·2023
Same author

Quantification of insulin-like growth factor-I by liquid chromatography-mass spectrometry.

Rapid communications in mass spectrometry : RCM·2022
Same author

A Targeted Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Quantification of Peptides from the Carboxyl-terminal Region of Type III Procollagen, Biomarkers of Collagen Turnover.

Clinical chemistry·2022
Same author

A miniaturized passive sampling-based workflow for monitoring chemicals of emerging concern in water.

The Science of the total environment·2022
Same journal

Intra- and inter-biosynthetic gene cluster allelic variation as drivers of chemical diversification in Streptomyces.

Essays in biochemistry·2026
Same journal

Considering internal conflict in the face of natural product biosynthesis and biosynthetic gene cluster evolution.

Essays in biochemistry·2026
Same journal

The plant holobiont: integrating molecular priming and ecological legacies for climate-adaptive immunity.

Essays in biochemistry·2026
Same journal

Bacterial-fungal interactions: connections and consequences.

Essays in biochemistry·2026
Same journal

Invasive plasmids as ecosystem engineers-from mechanism to application.

Essays in biochemistry·2026
Same journal

From early defence priming to lasting memory: developmental and seasonal dynamics in trees.

Essays in biochemistry·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Drug testing.

David A Cowan1

  • 1Drug Control Centre, Department of Forensic Science and Drug Monitoring, Pharmaceutical Science Division, King's College London, 150 Stamford Street, London SE1 9NH, UK. david.cowan@kcl.ac.uk

Essays in Biochemistry
|April 4, 2008
PubMed
Summary
This summary is machine-generated.

This chapter details advancements in sports anti-doping analysis, including isotope ratio MS and LC-MS/MS, to detect prohibited substances like testosterone and human growth hormone, ensuring fair play.

More Related Videos

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones
06:06

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Published on: February 5, 2018

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
11:14

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

Related Experiment Videos

Last Updated: Jul 6, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones
06:06

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Published on: February 5, 2018

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
11:14

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Sports Science

Background:

  • The detection of prohibited substances in sports has evolved significantly since the 1960s.
  • Modern analytical techniques are crucial for maintaining the integrity of sports.
  • The World Anti-Doping Agency (WADA) oversees the implementation of anti-doping tests.

Purpose of the Study:

  • To describe the latest techniques and applications in sports anti-doping analysis.
  • To highlight the role of WADA in enforcing anti-doping regulations.
  • To cover methods for detecting both small and large molecules, as well as blood doping.

Main Methods:

  • Gas Chromatography-Mass Spectrometry (GC-MS) for routine small molecule analysis.
  • Isotope Ratio Mass Spectrometry (IRMS) for detecting testosterone and nandrolone administration.
  • Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for diuretics, erythropoietin, and human growth hormone detection.
  • Immunoprocedures for large molecules like erythropoietin and human growth hormone.
  • Description of methods for detecting homologous blood transfusions.

Main Results:

  • Advanced techniques like IRMS and LC-MS/MS offer enhanced sensitivity and specificity for detecting prohibited substances.
  • LC-MS/MS is versatile, applicable to both small molecules (diuretics) and large molecules (hormones).
  • Immunoprocedures remain important for detecting large biomolecules.
  • New methods are available for detecting blood doping practices.

Conclusions:

  • Continuous technological innovation is essential for effective anti-doping efforts in sports.
  • A combination of advanced mass spectrometry and immunoassays provides comprehensive detection capabilities.
  • The evolution of analytical methods supports WADA's mission to ensure fair competition.