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XC-cell fusion induced by murine plasmocytoma cell. III. RNA gene expression correlated to syncytium formation
Abstract:
The MF2 strain, a mouse myeloma derived cell line, was found to continuously produce C-type viral particles when maintained in tissue-culture. These cells when cultured in an ascitic form by injection to Balb/c mice lost this property. The ability to induce syncytia by cocultivation of the MF2 cell with XC-cells was shown to be related to the viral production. A DNA complementary to viral 70 S RNA was synthesized using the viral reverse-transcriptase endogenous activity. The quality of the probe is discussed and the expression of the viral genome among cellular poly A rich RNA varied concomitently to the syncytium inducing ability as evidenced by molecular hybridization experiments.
Insights
Mouse myeloma cells (MF2) produce C-type viruses in culture but lose this ability in mice. Viral production correlates with syncytia induction and viral gene expression in RNA.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- MF2, a mouse myeloma cell line, was observed to produce C-type viral particles.
- This viral production was dependent on the culture conditions.
Purpose of the Study:
- To investigate the factors affecting C-type viral particle production in MF2 cells.
- To explore the relationship between viral production, syncytia induction, and viral gene expression.
Main Methods:
- MF2 cells were cultured in vitro and in vivo (ascitic form in Balb/c mice).
- Syncytia induction was assessed by co-cultivation with XC-cells.
- Complementary DNA (cDNA) was synthesized from viral 70S RNA using reverse transcriptase.
- Molecular hybridization was used to study viral genome expression in cellular poly(A)-rich RNA.
Main Results:
- MF2 cells lost the ability to produce C-type viral particles when cultured in an ascitic form in mice.
- Viral production was directly correlated with the ability to induce syncytia.
- The expression of the viral genome in cellular RNA varied with syncytia-inducing ability.
Conclusions:
- The study demonstrates a link between C-type viral production, syncytia formation, and viral gene expression in MF2 cells.
- Culture conditions significantly impact viral particle production in this cell line.