Related Experiment Video
Updated: Jul 6, 2026
05:16
Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research
Published on: October 27, 2023
An ultrastructural investigation of tissue-engineered pulp constructs implanted within endodontically treated teeth
Eric L Gotlieb1, Peter E Murray, Kenneth N Namerow
1Department of Endodontics, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Journal of the American Dental Association (1939)
|April 4, 2008
Summary
Tissue-engineered pulp constructs can be successfully implanted into cleaned and shaped root canals, demonstrating a proof-of-concept for future regenerative endodontic treatments using laboratory-created dental pulp tissue.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dental Research
Background:
- Investigating ultrastructural scanning electron microscopy (SEM) of tissue-engineered pulp constructs.
- Focus on constructs implanted within endodontically treated teeth.
Purpose of the Study:
- To evaluate the feasibility of implanting tissue-engineered pulp constructs into endodontically treated teeth.
- To assess cell adherence and construct integration using SEM.
Main Methods:
- Utilizing stem cells from human exfoliated deciduous teeth seeded on polylactic acid scaffolds.
- Incorporating bone morphogenic protein-2 and transforming growth factor beta1.
- Implanting constructs into 105 extracted human premolar teeth after cleaning and shaping.
Main Results:
- SEM analysis revealed successful cell adherence within all pulp constructs.
- Minimal differences observed between scaffold types or with growth factor addition.
- Demonstrated proof-of-concept for implanting engineered pulp into root canals.
Conclusions:
- Supports the possibility of implanting tissue-engineered pulp constructs post-cleaning and shaping.
- Highlights potential for future regenerative endodontic treatments.
- Envisions laboratory-created vital dental pulp tissue constructs for clinical application.
