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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
Published on: February 6, 2021
Toward improving therapeutic regimens for Bacillus endophthalmitis
Brandt J Wiskur1, Michael L Robinson, Allison J Farrand
1Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Investigative Ophthalmology & Visual Science
|April 4, 2008
Summary
Early intravitreal vancomycin or gatifloxacin improved outcomes for Bacillus cereus endophthalmitis. Dexamethasone addition offered no benefit and may reduce antibiotic efficacy, especially when treatment is delayed past six hours.
Area of Science:
- Ophthalmology
- Infectious Diseases
- Microbiology
Background:
- Bacillus cereus causes severe endophthalmitis.
- Effective treatment for B. cereus endophthalmitis is crucial.
Purpose of the Study:
- To evaluate early vancomycin or gatifloxacin treatment for experimental B. cereus endophthalmitis.
- To assess the impact of dexamethasone co-administration on treatment outcomes.
Main Methods:
- Intravitreal injection of B. cereus in rabbit eyes.
- Treatment with vancomycin, gatifloxacin, dexamethasone, or combinations at 2, 4, or 6 hours post-infection.
- Analysis via electroretinography, bacterial quantitation, and drug penetration studies.
Main Results:
- Early antibiotic treatment (2 hours) with or without dexamethasone improved retinal function.
- Antibiotic treatment alone at 6 hours showed better retinal function than antibiotic plus dexamethasone.
- Most antibiotic treatments sterilized eyes; gatifloxacin achieved therapeutic concentrations.
Conclusions:
- Early intravitreal vancomycin or gatifloxacin is effective for B. cereus endophthalmitis.
- Dexamethasone did not enhance therapeutic benefits and potentially reduced vancomycin efficacy.
- Treatment delay beyond 6 hours significantly impaired vision salvage.
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