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Published on: June 3, 2022
Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS
Melinda E Sanders1, Eduardo C Dias, Baogang J Xu
1Department of Pathology, Vanderbilt University, Nashville, Tennessee 37232, USA. melinda.sanders@vanderbilt.edu
Abstract:
We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.
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