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Related Experiment Videos

Navigating transdermal diffusion with multiphoton fluorescence lifetime imaging.

D K Bird1, A L Schneider, A C Watkinson

  • 1Ultrafast and Microspectroscopy Laboratories, School of Chemistry, The University of Melbourne, Victoria 3010, Australia. dbird@unimelb.edu.au

Journal of Microscopy
|April 5, 2008
PubMed
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Fluorescence lifetime imaging monitors pharmaceutical diffusion in human skin by detecting changes in intrinsic fluorophore lifetimes. This technique offers a novel way to study drug penetration and kinetics in the skin.

Area of Science:

  • Biophysics
  • Pharmacology
  • Dermatology

Background:

  • Transdermal drug delivery requires understanding pharmaceutical diffusion pathways and rates in human skin.
  • Current methods for monitoring diffusion are limited.
  • Intrinsic fluorophores in the stratum corneum offer potential biomarkers for monitoring diffusion.

Purpose of the Study:

  • To demonstrate fluorescence lifetime imaging by time-correlated single-photon counting (TCSPC) as a method for monitoring transdermal pharmaceutical diffusion.
  • To characterize fluorescence lifetimes in different human skin sections.
  • To investigate the effect of a drug formulation on fluorescence lifetimes.

Main Methods:

  • Utilized fluorescence lifetime imaging by time-correlated single-photon counting (TCSPC).

Related Experiment Videos

  • Analyzed intrinsic fluorophores in the intracellular regions between corneocytes of the stratum corneum.
  • Compared fluorescence lifetimes across dermatomed skin, epidermal membranes, and stratum corneum samples.
  • Applied a drug/solvent formulation (ethinyl estradiol in ethanol) to epidermal membrane sections.

    • Main Results:

    • Statistically significant differences in the short fluorescence lifetime component were observed between different skin sample types.
    • Application of the ethinyl estradiol/ethanol formulation resulted in a statistically significant shortening of the long-lived fluorescence lifetime from approximately 2.8 ns to 2.5 ns.
    • Demonstrated the sensitivity of fluorescence lifetime measurements to changes induced by surface-applied formulations.

    Conclusions:

    • Fluorescence lifetime imaging by TCSPC is a promising method for monitoring transdermal diffusion pathways and rates of pharmaceuticals.
    • The technique can detect subtle changes in skin's intrinsic fluorescence upon drug application.
    • This approach holds potential for future studies on the kinetics and pathways of various transdermal formulations.