Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Upgrading a microscope with a spiral phase plate.

C Maurer1, A Jesacher, S Fürhapter

  • 1Division for Biomedical Physics, Innsbruck Medical University Müllerstr, 44, A-6020 Innsbruck, Austria. christian.maurer@i-med.ac.at

Journal of Microscopy
|April 5, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Emergency room visits for asthma and the reported outcomes: The PASS survey by the CRISALIS network].

Revue des maladies respiratoires·2025
Same author

Treatment of a symptomatic cervical cerebrospinal fluid fistula after full endoscopic cervical foraminotomy with CT-guided epidural fibrin patch.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2023
Same author

The FRESH Study: Treatment of Intracranial Aneurysms with the New FRED X Flow Diverter with Antithrombotic Surface Treatment Technology-First Multicenter Experience in 161 Patients.

AJNR. American journal of neuroradiology·2023
Same author

Third international challenge to model the medium- to long-range transport of radioxenon to four Comprehensive Nuclear-Test-Ban Treaty monitoring stations.

Journal of environmental radioactivity·2022
Same author

[Spontaneous intracranial hypotension with brain sagging and reversible frontotemporal dementia : Case report and review of the literature].

Der Nervenarzt·2022
Same author

[Severe adult asthma and treatment adherence: Results of the FASE-CPHG study].

Revue des maladies respiratoires·2021
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

A novel spiral phase plate enhances contrast in standard bright-field microscopes for phase and amplitude samples. This technique improves spatial resolution and offers quantitative phase and amplitude information.

Area of Science:

  • Microscopy
  • Optical Imaging
  • Biophysics

Background:

  • Standard bright-field microscopy has limitations in visualizing phase and amplitude contrast.
  • Existing phase contrast methods require specialized setups.

Purpose of the Study:

  • To introduce a spiral phase plate for contrast enhancement in bright-field microscopy.
  • To demonstrate its applicability in various microscopy setups and its potential for improved spatial resolution.

Main Methods:

  • Implementation of a spiral phase plate in the Fourier plane of a standard bright-field microscope.
  • Utilizing illumination sources with partial spatial coherence.
  • Testing with transmissive, reflective, and epi-illumination sample configurations.

Related Experiment Videos

Main Results:

  • Significant contrast enhancement for phase and amplitude samples.
  • Theoretical improvement in spatial resolution for white light microscopy.
  • Generation of relief-like images similar to differential interference contrast microscopy with slight plate misalignment.

Conclusions:

  • The spiral phase plate is a versatile tool for enhancing contrast in microscopy.
  • It offers a practical alternative to standard phase contrast methods.
  • Numerical processing of generated images allows for quantitative phase and amplitude retrieval.