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Updated: Jul 6, 2026
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Murine Model of CD40-activation of B cells
Published on: March 5, 2010
Development and in vitro characterization of canine CD40-Ig
Christoph Jochum1, Mechthild Beste, Diane Stone
1Transplantation Biology Program, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA; Department of Gastroenterology & Hepatology, University of Duisburg-Essen, Germany.
Veterinary Immunology and Immunopathology
|April 5, 2008
Summary
Researchers developed a canine-specific CD40-Ig fusion protein to improve marrow transplantation outcomes. This new reagent demonstrated superior immunosuppressive effects compared to existing antibodies in canine mixed leukocyte reactions.
Area of Science:
- Immunology
- Transplantation Biology
- Protein Engineering
Background:
- Previous attempts using anti-CD154 antibody 5c8 showed limited success in canine marrow transplantation.
- A canine-specific reagent is needed to enhance engraftment and improve transplantation outcomes.
Purpose of the Study:
- To develop and evaluate a canine-specific CD40-Ig fusion protein for immunosuppression.
- To compare the effectiveness of canine CD40-Ig with the anti-CD154 antibody 5c8 in vitro.
Main Methods:
- Constructed a canine CD40-Ig fusion protein using mouse IgG2a Fc tail.
- Co-transfected DHFR-deficient CHO cells and selected stable, high-producing clones.
- Purified canine CD40-Ig and assessed its immunosuppressive activity in mixed leukocyte reactions.
Main Results:
- Confirmed the production of a canine CD40-Ig dimer via mass spectrometry.
- Canine CD40-Ig demonstrated potent immunosuppression, blocking mixed leukocyte reactions at 1nM.
- Canine CD40-Ig was 10 times more effective than the anti-CD154 antibody 5c8.
Conclusions:
- Canine CD40-Ig exhibits greater immunosuppressive capacity than anti-CD154 antibody 5c8 in canine mixed leukocyte reactions.
- This novel canine CD40-Ig fusion protein holds promise for improving outcomes in canine marrow transplantation models.
