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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Updated: Jul 6, 2026

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

Is MutaMouse insensitive to clastogens?

Anthony M Lynch1, Anuska G Mahabir, Andrea Bradford

  • 1GlaxoSmithKline, Department of Genetic Toxicology, Ware, UK.

Mutation Research
|April 5, 2008
PubMed
Summary
This summary is machine-generated.

The MutaMouse assay is insensitive to clastogens, but the peripheral blood micronucleus test detects genotoxicity from bleomycin and camptothecin. This suggests the micronucleus test complements transgenic gene mutation assays for genotoxicity testing.

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Area of Science:

  • Toxicology
  • Genetics
  • Biochemistry

Background:

  • MutaMouse is a transgenic rodent model used for genotoxicity testing.
  • Previous studies suggest MutaMouse is insensitive to clastogens, compounds that cause DNA breaks.
  • This insensitivity may limit its utility for detecting certain types of genotoxic agents.

Purpose of the Study:

  • To evaluate the sensitivity of MutaMouse to clastogenic agents.
  • To assess the utility of the peripheral blood micronucleus test as an adjunct to the MutaMouse assay.
  • To investigate the genotoxicity of bleomycin, camptothecin, m-AMSA, and o-AMSA.

Main Methods:

  • Dose range-finding studies were conducted in CD2F1 mice to determine appropriate dosing for MutaMouse studies.
  • Peripheral blood micronucleus tests (% MN-RET) were performed using flow cytometry.
  • MutaMouse mice were treated with bleomycin or camptothecin, and peripheral blood and tissues were analyzed for micronuclei and mutations.

Main Results:

  • Camptothecin was clastogenic in MutaMouse, but none of the tested compounds induced mutations at the lacZ locus.
  • Bleomycin induced micronuclei when administered intraperitoneally but not orally.
  • Both bleomycin and camptothecin increased micronucleated red blood cells in MutaMouse, while only bleomycin increased mutations in other tissues.

Conclusions:

  • MutaMouse demonstrates insensitivity to clastogens, aligning with previous findings.
  • The peripheral blood micronucleus test is a valuable complementary assay for detecting clastogenic genotoxicity.
  • Combining transgenic gene mutation assays with micronucleus tests provides a more comprehensive genotoxicity assessment.