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Related Experiment Videos

Serum-regulated nuclear localization is signal specific.

R H Lyons1

  • 1Department of Biological Chemistry and Reproductive Sciences, University of Michigan, Ann Arbor 48109-0404.

Molecular Endocrinology (Baltimore, Md.)
|December 1, 1991
PubMed
Summary
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Cellular proteins enter the nucleus via nuclear localization signals. This study reveals that the adenovirus E1a protein

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Virology

Background:

  • Nuclear factor activity is crucial for cellular processes.
  • Regulation of nuclear entry is a key mechanism for controlling protein function.
  • The precise mechanisms by which cells control nuclear import remain incompletely understood.

Purpose of the Study:

  • To investigate the serum-dependent regulation of nuclear localization.
  • To determine if the nuclear localization signal itself confers serum responsiveness.
  • To elucidate the role of the nuclear localization signal in adenovirus E1a protein's nuclear entry.

Main Methods:

  • Microinjection of adenovirus E1a protein into cultured cells.
  • Comparison of nuclear localization in serum-fed versus serum-starved cells.

Related Experiment Videos

  • Functional analysis of nuclear localization signals through substitution and transfer experiments.

    • Main Results:

    • Adenovirus E1a protein's nuclear entry is dependent on serum availability.
    • This serum responsiveness is an intrinsic property of the E1a nuclear localization signal.
    • Replacing the E1a signal with the SV40 T-antigen NLS abolishes serum dependence.
    • Transferring the E1a NLS to a heterologous protein confers serum-dependent localization.
    • Serum-induced nuclear import occurs rapidly, within 40 minutes of serum addition.

    Conclusions:

    • Nuclear accumulation of proteins is influenced by the properties of their nuclear localization signals.
    • The nature of the nuclear localization signal, not just its presence, dictates serum responsiveness.
    • This serum effect represents an early cellular response to growth factor signaling.