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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
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Related Experiment Video

Updated: Jul 6, 2026

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

Gene expression in human NAFLD.

Dario Greco1, Anna Kotronen, Jukka Westerbacka

  • 1Institute of Biotechnology, University of Helsinki, Finland.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|April 5, 2008
PubMed
Summary
This summary is machine-generated.

Nonalcoholic fatty liver disease (NAFLD) involves significant gene expression changes in the liver, impacting lipid and glucose metabolism, insulin signaling, and inflammation. These findings offer new insights into the pathogenesis of simple steatosis.

Related Experiment Videos

Last Updated: Jul 6, 2026

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

Area of Science:

  • Hepatology
  • Molecular Biology
  • Genomics

Background:

  • Nonalcoholic fatty liver disease (NAFLD) is prevalent, yet its pathogenesis remains poorly understood, especially in human liver tissues.
  • Studies on human liver samples are crucial for understanding NAFLD development.

Purpose of the Study:

  • To investigate global gene expression profiles in human livers with extreme steatosis due to NAFLD compared to those with low fat content.
  • To identify genes and pathways associated with the pathogenesis of simple steatosis.

Main Methods:

  • Utilized Affymetrix GeneChips to analyze gene expression of 17,601 genes in human liver samples.
  • Employed sequence-based reannotation and robust model-based normalization for data analysis.
  • Validated key gene expressions using real-time PCR.

Main Results:

  • Identified significant associations between liver fat content and genes involved in glucose/lipid metabolism, insulin signaling, inflammation, coagulation, and cell adhesion.
  • Discovered positive associations of ceramide signaling genes (MAP2K4, UGCG) with liver fat content.
  • Confirmed upregulation of genes related to lipid metabolism (PLIN, ACADM), fatty acid transport (FABP4, CD36), amino acid catabolism (BCAT1), and inflammation (CCL2) in high-fat livers.

Conclusions:

  • Simple steatosis in NAFLD is characterized by widespread alterations in hepatic gene expression.
  • These molecular changes highlight the complex interplay of metabolic and inflammatory pathways in NAFLD pathogenesis.