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Updated: Jul 6, 2026

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

Published on: June 2, 2023

Cardiac tissue engineering using perfusion bioreactor systems.

Milica Radisic¹, Anna Marsano, Robert Maidhof

¹Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.

Nature Protocols

|April 5, 2008

Summary

This summary is machine-generated.

This protocol engineers synchronously contractile cardiac tissue using biomimetic scaffolds and bioreactors. This method improves oxygen supply for cardiac cell culture, advancing tissue engineering applications.

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Last Updated: Jul 6, 2026

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

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Published on: June 2, 2023

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Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Published on: March 1, 2016

Area of Science:

Biomedical Engineering
Cardiovascular Research
Tissue Engineering

Background:

Conventional cardiac cell culture systems face limitations due to poor oxygen diffusion.
Developing functional cardiac constructs requires mimicking the in vivo microenvironment, including nutrient and oxygen supply.
Existing methods often fail to provide adequate oxygenation for complex tissue development.

Purpose of the Study:

To describe a protocol for engineering synchronously contractile cardiac constructs.
To enhance oxygen delivery to cultured cardiac cells using biomimetic approaches.
To provide a high-fidelity model for cardiac research and therapeutic development.

Main Methods:

Culturing cardiac cells on porous, channeled elastomer scaffolds within perfusion bioreactors.
Utilizing culture medium supplemented with oxygen carriers to mimic capillary networks.
Implementing a 2-4 week protocol including scaffold preparation, cell seeding, cultivation, and assessment.

Main Results:

Synchronously contractile cardiac constructs were successfully engineered.
The biomimetic approach significantly improved oxygen supply compared to conventional systems.
The protocol demonstrated suitability for human stem cell applications.

Conclusions:

The described protocol enables the tissue engineering of functional cardiac constructs with improved oxygenation.
This biomimetic strategy overcomes limitations of diffusional transport in standard cell culture.
The developed model serves as a valuable tool for cardiac research and regenerative medicine.