Related Experiment Video

Updated: Jul 6, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

[Start codon in the serine proteinase gene from Bacillus intermedius]

A R Kaiumov, A R Sabirova, N P Balaban

    Molekuliarnaia Biologiia
    |April 9, 2008
    PubMed

    More Related Videos

    Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes10:43

    Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

    Published on: October 3, 2019

    Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells09:14

    Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

    Published on: August 20, 2018

    Related Concept Videos

    Leaky Scanning02:28

    Leaky Scanning

    During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
    The Central Dogma01:25

    The Central Dogma

    Overview
    The Central Dogma01:20

    The Central Dogma

    The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
    RNA is the Missing Link Between DNA and Proteins
    In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
    From DNA to Protein03:06

    From DNA to Protein

    The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
    Initiation of Translation02:33

    Initiation of Translation

    Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
    First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
    Bacterial Transcription01:53

    Bacterial Transcription

    RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
    Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are: