Related Experiment Video
Updated: Jul 6, 2026
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Published on: September 19, 2016
[Cytokines of splenocytes in mice by different vaccination with recombinant BCG-Em14-3-3 vaccines against
Wen-gui Li1, Hong Wang, You-ming Zhu
1Institute of Infectious and Parasitic Diseases, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Objective:
To detect the changes of cytokines of splenocytes in mice immunized by recombinant BCG-Em14-3-3 vaccines against Echinococcus multilocularis (Em).
Methods:
BALB/c mice were subcutaneously and intranasally vaccinated by the BCG-Em14-3-3 vaccines respectively, with PBS as a control. The mice were challenged with Em protoscoleces eight weeks after the vaccinations. The spleens of the mice were collected 18 weeks after the infections. The splenocytes were separated and cultured with the stimulation of EmAg or ConA. The IL-2, IFN-gamma, TNF-alpha and IL-4 in the supernatants were measured with ELISA.
Results:
Significant increase of IFN-gamma and TNF-alpha were found in the immunized mice. But the IL-4 was decreased after the vaccinations. The levels of TNF-alpha were higher in the intranasal vaccinated mice than in the subcutaneous vaccinated mice.
Conclusions:
TH1 response is induced in mice by rBCG-Em14-3-3 vaccines to fight against the challenge of Em protoscoleces. Intranasal vaccinations are preferable.
