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Thermospray liquid chromatography-mass spectrometry for the characterisation of sulphate ester conjugates
G D Bowers1, D M Higton, G R Manchee
1Department of Biochemical Pharmacology, Glaxo Group Research Limited, Hertfordshire, UK.
Journal of Chromatography
|August 21, 1991
Abstract:
Formation of polar conjugates is a well documented metabolic pathway for xenobiotics containing phenolic hydroxyl groups. This paper describes the analysis of two sulphate ester conjugates by fast atom bombardment mass spectrometry and thermospray liquid chromatography-mass spectrometry. Thermospray liquid chromatography-mass spectrometry proved the more successful technique for obtaining the molecular weight of the intact conjugate, but only by removal of the buffer from the high-performance liquid chromatography eluent.