Incorporation of n-3 fatty acids into WB-F344 cell phospholipids inhibits gap junctional intercellular communication

In this investigation, we demonstrate that rat liver epithelial (WB-F344) cells grown in medium supplemented with n-3 fatty acids (FA) results in the inhibition of gap junctional intercellular communication (GJIC). Cells incubated for 48 hr in medium containing 50 microM alpha-linolenate (18:3n-3) resulted in a 60% inhibition of GJIC, compared to control cells, while treatment with gamma-linolenate (18:3n-6) had no effect. Supplementation with octadecatetraenoate (18:4n-3), eicosapentaenoate (20:5n-3), and docosahexaenoate (22:6n-3), inhibited GJIC by 42%, 28%, and 18%, respectively. Incubation with each of the n-3 FA markedly increased the total n-3 FA content of cellular phospholipids (PL). Growing cells in medium containing 50 microM arachidonate (20:4n-6) plus 50 microM 18:3n-3 partially attenuated the inhibition of GJIC induced by 18:3n-3. The mechanism by which n-3 FA inhibit GJIC remains to be determined.