[Preparation and characterization of McAbs against the human crosslinked fibrin degradation product D-dimer]

D-dimer is a specific degradation product of crosslinked fibrin hydrolyzed by plasmin. Detection of D-dimer in blood is important for the diagnosis of thrombotic diseases and the surveillance of thrombolytic treatment. Therefore, D-dimer purification and preparation of anti-D-dimer McAb are necessary tasks. Using routine methods, we prepared four hybridomas which secrete anti-D-dimer McAb: HID1, HID2, HID3 and HID4. After five months in culture these hybridomas still secrete anti-D-dimer McAb. The titer of ascites McAb is 1:10(-5)-1:10(-6). HID1, HID2, and HID3 recognize the same or similar antigenic determinants and don't cross-react with coagulation factors, plasma or fibrinogen degradation products X, Y, D, and E fragments. HID4 recognizes another antigenic determinant and cross-reacts with fibrinogen degradation products X, Y and D fragments. Immunoblot results show that all of these McAbs react with a MW 190,000 band. These antibodies are being used in the development of enzyme-linked and latex immunoassay methods for the detection of D-dimer and will be useful in the diagnosis, evaluation and monitoring of thrombotic diseases and thrombolytic treatment.