Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Interfacial Electrochemical Methods: Overview

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Meteor over New York City: Brines in a primitive CM asteroid.

Science advances·2026

Same author

Metabolomic profile of edible Amazonian Arecaceae fruits by FT-ICR-MS: Insights into chemical, nutritional and antioxidant profiles.

Food chemistry·2026

Same author

Highly dynamic metabolic response of grapevine to water deficits reveals an adaptability to a wide range of climatic conditions.

Food chemistry: X·2026

Same author

Mars Organic Geochemistry-Are We Alone, and Where Did We Come From?

Astrobiology·2026

Same author

High Relevance of Fatty Acid Oxidation in a Migrating Mammal, the Nathusius' Pipistrelle (Pipistrellus nathusii).

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Ozone alters the allergenicity of Ambrosia artemisiifolia pollen in a dose-dependent manner.

Environment international·2026

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Capillary electrochromatography and on-line concentration.

Guichen Ping¹, Philippe Schmitt-Kopplin, Yukui Zhang

¹Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|April 9, 2008

Summary

This study presents improved monolithic columns for capillary electrochromatography (CEC), a powerful separation technique. These columns enhance detection sensitivity by combining chromatographic and electric field effects for better analysis.

More Related Videos

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jul 6, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary electrochromatography (CEC) integrates capillary zone electrophoresis and high-performance liquid chromatography.
Monolithic stationary phases are ideal for CEC due to ease of preparation, frit elimination, and superior performance.

Purpose of the Study:

To present procedures for preparing improved monolithic CEC columns.
To demonstrate the separation of acidic and basic compounds using these columns.
To introduce an on-line concentration technique for enhanced CEC detection sensitivity.

Main Methods:

Preparation of monolithic CEC columns with a specific configuration (stationary phases flanking a detection window).
Demonstration of separation protocols for acidic and basic compounds.
Implementation of an on-line concentration technique leveraging simultaneous chromatographic zone sharpening and field-amplified sample stacking.

Main Results:

The improved monolithic columns facilitate effective separation of acidic and basic compounds.
The on-line concentration technique significantly enhances CEC detection sensitivity.
Simultaneous application of chromatographic and electric field effects is key to improved sensitivity.

Conclusions:

The presented monolithic column preparation offers an improved configuration for CEC.
The combined chromatographic zone sharpening and field-amplified sample stacking effectively boost CEC detection sensitivity.
This approach advances CEC as a sensitive micro-separation technique.