[Gene diagnosis of hemophilia A by PCR]

DNA fragments containing restriction enzyme Bcl I or XbaI polymorphic sites within the gene of coagulation factor VIII were amplified, respectively. Carrier detection and prenatal diagnosis of hemophilia A were performed by analysis of the polymorphisms of the amplified DNA. This is a simple, fast and sensitive technique, especially for prenatal diagnosis with very small amounts of chorionic villi or amniocytes. Four families with hemophilia A were analyzed, and 3 cases at high risk for hemophilia A were diagnosed prenatally.