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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
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A practical interface for microfluidics and nanoelectrospray mass spectrometry.

Sergio L S Freire1, Hao Yang, Aaron R Wheeler

  • 1Department of Chemistry, University of Toronto, Toronto, ON, Canada.

Electrophoresis
|April 9, 2008
PubMed
Summary

Researchers developed a novel method for fabricating nanospray ionization tips using glass and parylene-C. This technique enables rapid, batch production of identical, high-performance emitters for mass spectrometry (MS).

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A Microfluidic Chip for ICPMS Sample Introduction
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11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Microfabrication

Background:

  • Conventional nanospray ionization (NSI) emitters can be costly and difficult to fabricate consistently.
  • Integration of emitters with microfluidic devices often suffers from dead volumes, impacting separation efficiency.
  • There is a need for robust, reproducible, and easily manufactured NSI tips compatible with microfluidic systems.

Purpose of the Study:

  • To develop a novel, cost-effective method for fabricating integrated nanospray ionization tips.
  • To demonstrate the compatibility of these emitters with microfluidic separations.
  • To evaluate the performance of the fabricated emitters with diverse analytes.

Main Methods:

  • Fabrication of nanospray ionization tips using photolithography on glass substrates coated with parylene-C.
  • Integration of emitters contiguously with microchannels to eliminate dead volumes.
  • Characterization of device performance using various analytes including synthetic polymers, peptides, and nucleic acids.

Main Results:

  • Successful fabrication of thin (0.3 mm) devices with integrated emitters and microchannels using a single photolithography step.
  • Elimination of dead volumes in the fabricated emitter-microchannel system.
  • Nanospray ionization performance comparable to conventional emitters for diverse analytes.
  • Demonstrated rapid, batch fabrication of identical devices.

Conclusions:

  • The developed photolithography method offers a rapid and scalable approach for producing high-performance nanospray ionization tips.
  • The integrated design minimizes dead volumes, making it suitable for microfluidic applications.
  • These novel emitters provide a cost-effective and reproducible alternative to conventional NSI tips for mass spectrometry.