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Updated: Jul 6, 2026

Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions
Published on: April 17, 2026
Determination of analyte concentration using the residual solvent resonance in (1)H NMR spectroscopy
Gregory K Pierens1, Anthony R Carroll, Rohan A Davis
1Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, QLD, Australia. Greg.Pierens@cmr.uq.edu.au
Abstract:
An NMR protocol that uses the residual proton signal from DMSO -d(6) (i.e., DMSO -d(5)) to determine the concentration of an analyte in a NMR sample was developed. This technique provides an alternative method for determining the molar concentration of compounds in solution without prior knowledge of their molecular weight. The method is particularly useful when submilligram quantities of compound are to be analyzed and is applicable to a variety of different research areas such as compound management, and natural product, combinatorial, and medicinal chemistry.
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