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Optical biosensor based on hollow integrated waveguides.

Víctor J Cadarso1, César Fernández-Sánchez, Andreu Llobera

  • 1Institut de Microelectronica de Barcelona, CNM-IMB, CSIC, Campus UAB 08193 Barcelona, Spain.

Analytical Chemistry
|April 9, 2008
PubMed
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This study introduces a novel silicon waveguide biosensor for detecting hydrogen peroxide using immobilized enzymes. The cost-effective, miniaturized platform demonstrates high sensitivity and linear response, paving the way for advanced lab-on-a-chip systems.

Area of Science:

  • Integrated optics
  • Biosensor technology
  • Silicon photonics

Background:

  • Biosensors are crucial for detecting various analytes.
  • Silicon photonics offers a platform for miniaturized optical devices.
  • Enzyme immobilization is key for biosensor stability and reusability.

Purpose of the Study:

  • To develop and characterize the first absorbance biosensor utilizing pure silicon hollow integrated waveguides.
  • To create an enzymatic sensor for hydrogen peroxide (H2O2) measurement.
  • To demonstrate the potential of this platform for lab-on-a-chip applications.

Main Methods:

  • Fabrication of silicon hollow integrated waveguides using photolithography.
  • Covalent immobilization of horseradish peroxidase (HRP) via silanization chemistry.

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  • Numerical simulation and experimental characterization of sensor performance, including sensitivity and linear range.

    • Main Results:

    • Achieved linear sensor response for H2O2 detection up to 300 microM.
    • Demonstrated a sensitivity of 3 x 10(-3) AU/microM with a signal-to-noise ratio of approximately 20 dB.
    • Determined an apparent Michaelis-Menten constant of 0.44 mM for the immobilized enzyme.

    Conclusions:

    • The developed silicon waveguide biosensor is cost-effective, easy-to-use, and miniaturized.
    • The technology is suitable for developing generic biosensor platforms for lab-on-a-chip systems.
    • Experimental results align well with simulation predictions, validating the sensor design.