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Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
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Published on: April 20, 2015

Membrane localization of the MAK-V protein kinase

S V Kalinichenko1, E V Korobko, I V Korobko

  • 1Institute of Gene Biology, Russian Academy of Sciences, ul. Vavilova 34/5, Moscow, Russia.

Biochemistry. Biokhimiia
|April 9, 2008
PubMed

Insights

MAK-V, an AMPK-like protein kinase, is significantly associated with cellular membranes in mammalian cells. This membrane targeting appears conserved across species, suggesting a fundamental role in MAK-V function.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Protein kinase activity is often regulated by compartmentalization within cells.
  • MAK-V is an AMPK-like protein kinase with largely unknown functions and regulation.
  • Intracellular localization is crucial for protein kinase function.

Purpose of the Study:

  • To investigate the subcellular localization of MAK-V protein in mammalian cells.
  • To determine if MAK-V's membrane association is conserved across species.
  • To elucidate the mechanism behind MAK-V's association with intracellular membranes.

Main Methods:

  • Biochemical cell fractionation of mammalian cells to analyze MAK-V distribution.
  • Expression of MAK-V in yeast to assess evolutionary conservation of localization.
  • Analysis of MAK-V deletion mutants and cross-species sequence comparison.

Main Results:

  • A substantial amount of MAK-V protein in mammalian cells is found associated with cellular membranes.
  • MAK-V expressed in yeast also exhibits membrane targeting, indicating an evolutionarily conserved mechanism.
  • Specific MAK-V deletion mutants and sequence features were identified as potentially involved in membrane association.

Conclusions:

  • MAK-V protein demonstrates significant association with intracellular membranes.
  • The membrane targeting of MAK-V is an evolutionarily conserved process.
  • A potential mechanism for MAK-V's membrane localization has been proposed based on mutant and sequence analyses.

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