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A genomic sequence of the Schizosaccharomyces pombe 16 kDa vacuolar H(+)-ATPase
R Toyama1, D J Goldstein, R Schlegel
1Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
Yeast (Chichester, England)
|December 1, 1991
Abstract:
We have isolated the gene encoding the 16 kDa vacuolar H(+)-ATPase from Schizosaccharomyces pombe. On the basis of RNA splicing signals and amino acid sequence homology with other 16 kDa H(+)-ATPases, the genomic DNA sequence indicated the 16 kDa protein is encoded by five exons. The C-terminal 50 amino acids has more than 90% homology with vacuolar H(+)-ATPases of mammalian cells.