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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Related Experiment Video

Updated: Jul 6, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

A mobility shift detection method for DNA methylation analysis using phosphate affinity polyacrylamide gel

Emiko Kinoshita-Kikuta1, Eiji Kinoshita, Tohru Koike

  • 1Department of Functional Molecular Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan.

Analytical Biochemistry
|April 9, 2008
PubMed
Summary

This study introduces a new DNA methylation analysis method combining bisulfite conversion, methylation-specific PCR (MSP), and Phos-tag PAGE. The technique successfully detected methylated cytosine in a pUC19 plasmid.

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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Related Experiment Videos

Last Updated: Jul 6, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • DNA methylation is a critical epigenetic modification influencing gene expression.
  • Accurate detection of DNA methylation is essential for understanding various biological processes and diseases.
  • Existing methods for DNA methylation analysis can be complex or lack sensitivity.

Purpose of the Study:

  • To develop a novel, sensitive, and efficient procedure for DNA methylation analysis.
  • To enable the detection of methylated cytosine bases in DNA samples.
  • To establish a method combining established techniques with a new detection strategy.

Main Methods:

  • Utilized bisulfite-mediated cytosine-to-uracil conversion for DNA modification.
  • Employed methylation-specific polymerase chain reaction (MSP) with modified primers.
  • Integrated phosphate affinity polyacrylamide gel electrophoresis (PAGE) using Zn(2+)-Phos-tag for enhanced detection.

Main Results:

  • The developed procedure successfully detected methylated cytosine in a pUC19 plasmid.
  • The method demonstrated a mobility shift in PAGE, distinguishing methylated from unmethylated DNA.
  • The combination of MSP and Phos-tag PAGE provided a sensitive detection mechanism.

Conclusions:

  • The described procedure offers a novel approach for DNA methylation analysis.
  • This method enables sensitive detection of methylated cytosine bases.
  • The technique holds potential for various applications in epigenetics research and diagnostics.