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[Isolation and purification of human brain creatine kinase (CK-BB)]
Z Lu1
1Institute of Cardiovascular Diseases, Beijing.
Abstract:
A improved method for isolation and purification of CK-BB from human brain is introduced. CK-BB of fraction IV was purified to approximately 306.9 fold as compared with homogenate of human brain. The specific activity was 322.2 IU/mg protein. The recovery of creatine kinase activity was 52.8%. This fraction was analyzed by SDS-PAGE. It showed a single major band with an estimated molecular weight of 43000 when it stained with coomassie blue. Purity of fraction V was much better than fraction IV, but some CK activity was lost.