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Updated: Jul 6, 2026
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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
Published on: September 9, 2011
Vascular prostheses: performance related to cell-shear responses.
Kirstie D Andrews1, Patrick Feugier, Richard A Black
1UKCTE, Division of Clinical Engineering, Duncan Building, University of Liverpool, Liverpool, United Kingdom. k.d.andrews@liv.ac.uk
The Journal of Surgical Research
|April 9, 2008
Summary
Vascular biomaterial choice significantly impacts cell behavior and adhesion. Fibrous polyurethane (PU) showed the most promise for improving vascular prosthesis functionality under shear stress.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Investigating endothelialization of vascular prostheses for improved tissue engineering outcomes.
- Understanding initial cellular behavior on biomaterials before exposure to physiological shear stress.
Purpose of the Study:
- To examine pre-shear stress cellular responses on three vascular biomaterials.
- To correlate initial cell behavior with subsequent responses to shear stress.
Main Methods:
- Cell seeding of expanded polytetrafluoroethylene (ePTFE), polyester (PET), and polyurethane (PU) with L929 fibroblasts or HUVECs.
- Analysis of cytoskeletal involvement, cell height, and immunohistochemistry after 7 days of static culture.
Main Results:
- Differential cell behavior observed between biomaterials and cell types.
- Higher cytoskeletal involvement on fibrous surfaces and with HUVECs.
- Varied expression of adhesion molecules (ICAM-1, E-selectin, VCAM-1) across materials and cell types.
Conclusions:
- Material substrate significantly influences cellular response and adhesion.
- Enhanced cell adhesion correlates with increased cytoskeletal processes and adhesion marker expression.
- Fibrous PU demonstrated suitability for shear stress exposure, impacting long-term prosthesis function.
