Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

TIEG-null mice display an osteopenic gender-specific phenotype.

J R Hawse¹, U T Iwaniec, S F Bensamoun

¹Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.

|April 9, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Probable obsessive-compulsive disorder among youth in Singapore: Prevalence, sociodemographic correlates, and the association with psychological distress, harmful alcohol use and functional impairment.

Comprehensive psychiatry·2026

Same author

Family History and Solar Insolation in Bipolar I Disorder.

Acta psychiatrica Scandinavica·2026

Same author

Corrigendum to "Dose-response effects of alcohol on biochemical markers of bone turnover in non-human primates: Effects of species, sex and age of onset of drinking" [Bone Rep. 16 (2021) 101159].

Bone reports·2025

Same author

Corrigendum to "Between-subject and within-subject variability in measures of biochemical markers of bone turnover in cynomolgus and rhesus macaques" [Bone Rep. 15(2021) 101126].

Bone reports·2025

Same author

Examining psychological distress among youth in Singapore: Insights from the National Youth Mental Health Study.

Asian journal of psychiatry·2025

Same author

Multiscale analysis of Klf10's impact on the passive mechanical properties of murine skeletal muscle.

Journal of the mechanical behavior of biomedical materials·2023

TGFbeta inducible early gene-1 (TIEG) deficiency causes osteopenia in female mice by reducing bone formation. TIEG(-/-) female mice exhibit decreased bone mineral density and strength, impacting osteoblast function.

Area of Science:

Skeletal Biology
Molecular Endocrinology
Bone Physiology

Background:

TGFbeta inducible early gene-1 (TIEG) is crucial for osteoblast (OB) function and skeletal development.
Understanding TIEG's role is vital for skeletal health research.

Purpose of the Study:

To investigate the skeletal role of TIEG by generating and analyzing TIEG-null (TIEG(-/-)) mice.
To characterize the bone phenotype of TIEG(-/-) mice in a pure C57BL/6 background.

Main Methods:

Generation of congenic TIEG(-/-) mice.
Dual-energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT) analysis.
Mechanical 3-point bending tests, micro-CT, and histomorphometric analyses.

Main Results:

More Related Videos

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Published on: September 8, 2023

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Related Experiment Videos

Last Updated: Jul 6, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Trabecular Bone Microarchitecture Evaluation in an Osteoporosis Mouse Model

Published on: September 8, 2023

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Female TIEG(-/-) mice showed decreased bone mineral content, density, and area in femurs and tibias compared to wild-type (WT) littermates.
Reduced femur strength and increased flexibility were observed in female TIEG(-/-) mice.
Histomorphometry revealed decreased cancellous bone area, reduced trabecular number, and a 42% decrease in bone formation rate in female TIEG(-/-) mice.

Conclusions:

TIEG deficiency leads to osteopenia in female mice, primarily due to reduced bone formation.
The findings highlight TIEG's critical role in maintaining bone mass and strength in females.
Reduced functional/mature osteoblast numbers contribute to the observed osteopenic phenotype.