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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
Published on: March 7, 2019
Molybdate transport through the plant sulfate transporter SHST1
Kate L Fitzpatrick1, Stephen D Tyerman, Brent N Kaiser
1School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus Urrbrae, SA 5064, Australia.
Abstract:
Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1, on media containing low amounts of molybdate. Kinetic analysis using 99MoO4(2-) demonstrated that SHST1 enhanced the uptake of molybdate into yeast cells at nM concentrations. Uptake was not inhibited by sulfate, but sulfate transport via SHST1 was reduced with molybdate. These results are the first measurement of molybdate transport by a characterised plant sulfate transport protein.
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