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Automated, High-Throughput Detection of Bacterial Adherence to Host Cells
07:21

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Published on: September 17, 2021

LamB-mediated adherence of enteropathogenic Escherichia coli to HEp-2 cells

K Subramanian1, R B Shankar, S Meenakshisundaram

  • 1Centre for Biotechnology, Anna University, Chennai, India.

Journal of Applied Microbiology
|April 10, 2008
PubMed
Abstract

Insights

Maltoporin (LamB) acts as an adherence factor for enteropathogenic Escherichia coli (EPEC). This study demonstrates LamB

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Adherence is crucial for the pathogenesis of enteropathogenic Escherichia coli (EPEC).
  • The role of specific bacterial surface proteins in EPEC adherence requires further elucidation.

Purpose of the Study:

  • To investigate the function of maltoporin (LamB) as an adherence factor for EPEC.
  • To determine if LamB contributes to the in vitro adherence of EPEC to epithelial cells.

Main Methods:

  • Compared adherence of wild-type EPEC, a LamB mutant (DeltalamB), and a complemented strain to HEp-2 cells.
  • Utilized Western blotting to confirm antibody specificity against LamB.
  • Assessed adherence using latex beads coated with purified LamB protein and anti-LamB antibodies.

Main Results:

  • The DeltalamB mutant showed significantly reduced adherence compared to wild-type EPEC.
  • Complementation with the lamB gene restored adherence in the mutant strain.
  • Anti-LamB antibodies inhibited both bacterial and bead adherence, confirming LamB's role.

Conclusions:

  • Maltoporin (LamB) functions as an alternative or additional adherence factor for EPEC.
  • LamB represents a potential vaccine candidate for controlling EPEC infections due to its role in adherence and co-expression with virulence factors.

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