A methodology for measurements of nasal nitric oxide in children under 5 yr

Measurements of nasal nitric oxide (nNO) may give insight into respiratory conditions in children aged under 5 yr but no methodology has been described for this age-group. The present study aimed to establish the methodology and reproducibility for measuring nNO during tidal breathing in young children and to relate nNO to allergic conditions. Children and siblings aged under 5 yr attending hospital clinics were enrolled. On-line nNO measurements were obtained during tidal breathing using a chemiluminescence analyser. To establish our methodology, nNO was measured over 3, 5 or 10 s NO plateaus and nNO was also measured from left and right nostrils. nNO was then compared between children with and without allergic conditions. The reproducibility of nNO measurements over 24 h was studied in a separate group of children. Eighty-three children participated in the methodological part of the study and nNO was successfully measured in 57 (69%), mean (s.d.) age 3.4 (1.1) years, 14 with allergic conditions. Neither NO plateau duration nor choice of nostril influenced nNO values. The mean (s.d.) nNO for non-atopic children was 208 (103) parts per billion (ppb) and for atopic children was 284 (122), p = 0.032. Nasal NO values were not related to ambient NO, gender and passive smoke exposure; there was a non-significant trend for nNO to be positively related to age. Nasal NO measurements were reproducible in the 21 children tested, mean difference 9.6 ppb (limits of agreement-127, 146). We report a methodology for nNO measurement in young children. Further work is now required to establish the clinical utility of nNO in this age-group.