Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.1K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.1K
Nucleic Acids02:43

Nucleic Acids

50.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
50.6K
Nucleic acids02:43

Nucleic acids

190.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
190.0K
Nucleic Acids02:43

Nucleic Acids

9.0K
No description available
9.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.2K
Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

14.8K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifunctional Molecular Beacon Micelles for Intracellular mRNA Imaging and Synergistic Therapy in Multidrug-Resistant Cancer Cells.

Advanced functional materials·2017
Same author

Thiol-ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles.

Chemical science·2017
Same author

An effective thermal therapy against cancer using an E-jet 3D-printing method to prepare implantable magnetocaloric mats.

Journal of biomedical materials research. Part B, Applied biomaterials·2017
Same author

Control of cell growth on 3D-printed cell culture platforms for tissue engineering.

Journal of biomedical materials research. Part A·2017
Same author

Corrigendum: In situ targeted MRI detection of Helicobacter pylori with stable magnetic graphitic nanocapsules.

Nature communications·2017
Same author

In vivo imaging of alkaline phosphatase in tumor-bearing mouse model by a promising near-infrared fluorescent probe.

Talanta·2017

Related Experiment Video

Updated: Feb 6, 2026

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.7K

Molecular assembly for high-performance bivalent nucleic acid inhibitor.

Youngmi Kim1, Zehui Cao, Weihong Tan

  • 1Center for Research at the Bio/nano Interface, Department of Chemistry, University of Florida Genetics Institute, Shands Cancer Center, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 10, 2008
PubMed
Summary

Researchers created a bivalent nucleic acid ligand that acts as a potent protein inhibitor. This novel design significantly enhances binding affinity and offers a promising new anticoagulant therapy.

More Related Videos

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.7K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.1K

Related Experiment Videos

Last Updated: Feb 6, 2026

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.7K
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

8.7K
NanoDrop Microvolume Quantitation of Nucleic Acids
09:28

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

207.1K

Area of Science:

  • Biomolecular Engineering
  • Nucleic Acid Therapeutics
  • Protein-Ligand Interactions

Background:

  • Multivalent interactions offer enhanced affinity and selectivity over monovalent interactions in ligand design.
  • Biomolecular engineers utilize multivalent interactions to create advanced molecular assemblies and improve ligand performance.
  • While small molecules and antibody epitopes are common, nucleic acid aptamers are underutilized in designing multifunctional ligands.

Purpose of the Study:

  • To explore the design of bivalent nucleic acid ligands using thrombin and its aptamers as a model system.
  • To evaluate the functional performance of assembled nucleic acid ligands compared to monovalent counterparts.
  • To develop a high-performance bivalent protein inhibitor based on nucleic acid aptamers.

Main Methods:

  • Assembling two thrombin-binding aptamers with optimized design parameters.
  • Evaluating the inhibitory efficiency and binding kinetics of the bivalent aptamer assembly.
  • Testing the anticoagulant properties of the developed aptamer-based inhibitor in various samples.

Main Results:

  • Successful development of a nucleic acid-based high-performance bivalent protein inhibitor.
  • Achieved 16.6-fold greater inhibition efficiency compared to monovalent ligands due to simultaneous dual aptamer binding.
  • Demonstrated a significantly slower dissociation rate (k(off) approximately 1/50th), indicating enhanced binding stability.
  • Validated the aptamer assembly as an effective anticoagulant reagent.

Conclusions:

  • The optimized bivalent aptamer design provides a simple, noninvasive method for enhancing aptamer performance.
  • This approach holds significant promise for developing potent antithrombin agents for diseases involving abnormal thrombin activity.
  • The study highlights the potential of nucleic acid aptamers in creating advanced therapeutic molecules.