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Published on: March 11, 2018
[Reducing oxidative DNA damage by adding antioxidants in human semen samples undergoing cryopreservation procedure]
Zhi-ling Li1, Qiong-lin Lin, Rong-ju Liu
1Reproductive Center, First Affiliated Hospital of Medical College, Shantou University, Shantou, China.
Zhonghua Yi Xue Za Zhi
|April 11, 2008
Summary
Adding antioxidants like ascorbate and catalase (CAT) to human semen samples during cryopreservation significantly reduces oxidative DNA damage and improves sperm quality. This enhances sperm motility and viability post-freeze-thawing.
Area of Science:
- Reproductive Biology
- Oxidative Stress Research
- Cryopreservation Science
Context:
- Cryopreservation of human semen is crucial for assisted reproductive technologies.
- Oxidative stress during cryopreservation can lead to sperm DNA damage and reduced fertility.
- Antioxidants are explored to mitigate these negative effects.
Purpose:
- To evaluate the protective effects of ascorbate, catalase (CAT), and superoxide dismutase (SOD) against oxidative DNA damage in human semen during cryopreservation.
- To assess the impact of these antioxidants on sperm quality parameters, including motility, viability, and DNA integrity.
Summary:
- Human semen samples were cryopreserved with varying concentrations of ascorbate, CAT, and SOD.
- Comet assay and flow cytometry measured DNA damage and reactive oxygen species (ROS).
- Ascorbate (300 µmol/L) and CAT (200-400 U/ml) significantly reduced ROS levels, improved sperm motility (a+b grade), viable recovery rates, and preserved DNA integrity (TD% and OTM) compared to controls.
Impact:
- Ascorbate and CAT supplementation effectively protect human sperm DNA from oxidative damage during cryopreservation.
- These antioxidants improve key sperm quality parameters, enhancing the viability and potential fertility of cryopreserved samples.
- Findings support the use of specific antioxidants to optimize sperm cryopreservation protocols.
