Optimizing the application of entomopathogenic nematodes: experimental set-up

Area of Science:

• Agricultural Entomology
• Pest Management
• Nematology

Background:

• Optimizing spray application of Entomopathogenic Nematodes (EPNs) is crucial for effective biological pest control.
• Existing methods for evaluating EPN viability and deposition during spraying are often inadequate or time-consuming.

Purpose of the Study:

• To investigate the impact of hydraulic sprayer components on the viability and deposition of EPNs.
• To develop and validate an efficient image analysis system for assessing nematode viability.

Main Methods:

• Development of a modular spray application system for controlled EPN spraying experiments.
• Utilizing image analysis for nematode viability assessment, compared against traditional microscopic counting.
• Evaluation of different sprayer parts, including pump type, mixing system, nozzle type, and filter size.

Main Results:

• The modular system allows for systematic evaluation of sprayer effects on EPNs.
• The developed image analysis technique provides a viable alternative to standard microscopic counting.
• Experimental data will inform recommendations for optimal EPN spray application.

Conclusions:

• A novel modular spray system and image analysis technique have been established for EPN research.
• Further research using this system will yield practical guidelines for improving EPN spray application efficacy.
• This work addresses a significant gap in the practical application of biological control agents.