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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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Published on: July 3, 2016

Nutrition and mutagenesis

Lynnette R Ferguson1, Martin Philpott

  • 1Discipline of Nutrition, Faculty of Medical and Health Sciences, The University of Auckland, New Zealand. l.ferguson@auckland.ac.nz

Annual Review of Nutrition
|April 11, 2008
PubMed

Insights

Diet-related mutagens can cause DNA damage, leading to chronic diseases like cancer. Understanding these mutagens and antimutagens is key to preventing diet-related diseases through nutrigenomics.

Area of Science:

  • Toxicology
  • Genetics
  • Nutritional Science

Background:

  • Diet-related mutagens contribute to chronic diseases, including cancer and cardiovascular disease.
  • Many dietary mutagens are DNA-reactive, causing mutations and chromosomal changes.
  • Genomic instability can also arise from nutrient deficiencies.

Purpose of the Study:

  • To review the role of diet-related mutagens in disease etiology.
  • To discuss mechanisms of mutagenesis and antimutagenesis.
  • To highlight the importance of nutrigenomics in addressing diet-related risks.

Main Methods:

  • Review of scientific literature on dietary mutagens and their effects.
  • Identification of specific mutagenic compounds and their mechanisms of action.
  • Discussion of antimutagenic compounds and their protective roles.

Main Results:

  • Identified numerous dietary mutagens (e.g., aflatoxin B1, heterocyclic amines, polycyclic aromatic hydrocarbons) that induce DNA mutations.
  • Highlighted endogenous reactive species and inhibitors of critical cellular processes as additional mutagenic factors.
  • Noted that inadequate nutrient intake (e.g., folate, selenium) contributes to genomic instability.
  • Listed various antimutagens (e.g., vitamin C, polyphenols) that counteract mutagenic effects through diverse mechanisms.

Conclusions:

  • Diet-induced mutagenesis is a significant factor in chronic disease development.
  • Genetic variations influence individual susceptibility to diet-related diseases.
  • Nutrigenomic and nutrigenetic approaches are crucial for quantifying and mitigating diet-related mutagenesis.

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