A novel bradykinin potentiating peptide isolated from Bothrops jararacussu venom using catallytically inactive
Vanessa Rioli1, Benedito C Prezoto, Katsuhiro Konno
1Laboratório Especial de Toxinologia Aplicada-CAT/CEPID, Instituto Butantan, São Paulo, Brazil. vrioli@butantan.gov.br
The FEBS Journal
|April 11, 2008
Abstract:
Characterization of the peptide content of venoms has a number of potential benefits for basic research, clinical diagnosis, development of new therapeutic agents, and production of antiserum. Here, we use a substrate-capture assay that employs a catalytically inactive mutant of thimet oligopeptidase (EC 3.4.24.15; EP24.15) to identify novel bioactive peptides in Bothrops jararacussu venom. Of the peptides captured with inactive EP24.15 and identified by mass spectrometry, three were previously identified bradykinin-potentiating peptides (BPP),
