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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Cytokine signaling modules in inflammatory responses.
John J O'Shea1, Peter J Murray
1Molecular Immunology and Inflammation Branch, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20852, USA. osheajo@mail.nih.gov
Immunity
|April 11, 2008
Summary
Cytokine signaling through Jak-Stat pathways regulates inflammation in T cells, neutrophils, and macrophages. Suppressor of cytokine signaling (Socs) proteins negatively control these pathways, influencing diverse inflammatory outcomes.
Area of Science:
- Immunology
- Cellular Biology
- Molecular Signaling
Background:
- Cytokine signaling is crucial for regulating inflammatory responses.
- Janus kinase-Signal transducer and activator of transcription (Jak-Stat) pathways are key mediators of cytokine signaling.
- Suppressor of cytokine signaling (Socs) proteins are critical negative regulators of Jak-Stat pathways.
Purpose of the Study:
- To summarize the role of Jak-Stat signaling in T cells, neutrophils, and macrophages during inflammation.
- To highlight the regulatory function of Socs proteins in these Jak-Stat pathways.
- To emphasize the context-dependent outcomes of Jak-Stat-Socs signaling in inflammation.
Main Methods:
- Review of existing literature on cytokine signaling, Jak-Stat pathways, and Socs proteins.
- Focus on the molecular mechanisms of Jak-Stat-Socs regulation in immune cells.
- Analysis of how receptor activation and gene accessibility influence signaling outcomes.
Main Results:
- Jak-Stat pathways are central to immune cell function in inflammation.
- Socs proteins provide negative feedback, modulating pathway activity.
- The diverse outcomes of Jak-Stat-Socs signaling depend on specific cytokine receptors and cellular context.
Conclusions:
- Understanding Jak-Stat-Socs signaling is vital for comprehending inflammatory processes.
- Dysregulation of these pathways is linked to human inflammatory diseases.
- Targeting cytokine signaling offers therapeutic potential for managing inflammation.
Related Concept Videos
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