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Published on: January 7, 2019
Murine neutrophils present Class II restricted antigen
Shauna Culshaw1, Owain R Millington, James M Brewer
1Centre for Rheumatic Diseases, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, UK. s.culshaw@dental.gla.ac.uk
Immunology Letters
|April 11, 2008
Summary
Murine neutrophils can present antigens via MHC Class II, activating CD4+ T cells and promoting inflammation. This confirms human neutrophil findings and reveals a new pro-inflammatory role for these immune cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Neutrophils were traditionally viewed as innate immune cells.
- Emerging evidence suggests neutrophils influence adaptive immunity and may act as antigen-presenting cells.
- Human neutrophils express MHC Class II and costimulatory molecules under inflammatory conditions.
Purpose of the Study:
- To investigate neutrophil-T cell interactions in a murine model.
- To determine if murine neutrophils can present MHC Class II-restricted peptides to CD4+ T cells.
- To explore the pro-inflammatory effects of neutrophils in adaptive immune responses.
Main Methods:
- Utilized a murine T cell hybridoma with a transgenic T cell receptor specific for ovalbumin peptide 323-339 (OVA(323-339)) and a green fluorescent reporter.
- Isolated murine neutrophils and loaded them with OVA(323-339).
- Co-cultured antigen-pulsed neutrophils with OVA(323-339)-specific CD4+ T cells to assess T cell activation, proliferation, and cytokine production.
Main Results:
- Murine neutrophils loaded with OVA(323-339) induced T cell activation, confirmed by green fluorescent protein reporter expression.
- Antigen-pulsed neutrophils primed naive OVA-specific CD4+ T cells in a contact-dependent manner.
- Demonstrated T cell proliferation and cytokine production, indicating a pro-inflammatory role for murine neutrophils.
Conclusions:
- Murine neutrophils present MHC Class II-restricted peptides, activating CD4+ T cells.
- These findings confirm similar functions observed in human neutrophils.
- This study highlights a novel pro-inflammatory function of murine neutrophils in adaptive immunity.
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