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Related Experiment Video

Updated: Jul 6, 2026

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
04:53

Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles

Published on: May 26, 2023

A bioassay for metals utilizing a human cell line.

J Shea1, T Moran, P F Dehn

  • 1Department of Biology, Canisius College, Buffalo, NY 14208, USA.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|April 11, 2008
PubMed
Summary
This summary is machine-generated.

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This study shows the HepG2 human cell line effectively detects metal contamination in sediments using metallothionein (MT) as a biomarker. Results indicate cadmium is the primary inducer of MT in Lake Erie sediment extracts.

Area of Science:

  • Environmental Toxicology
  • Cell Biology
  • Biomarker Development

Background:

  • Aquatic sediments can accumulate toxic metals, posing risks to ecosystems and human health.
  • Effective bioassays are needed to monitor metal contamination and its biological impact.
  • Metallothionein (MT) is a protein known to bind metals and serve as a biomarker for metal exposure.

Purpose of the Study:

  • To evaluate the HepG2 human cell line as a bioassay for detecting metal contamination in sediments.
  • To use metallothionein (MT) induction as a biomarker for metal exposure in HepG2 cells.
  • To identify the specific metals responsible for MT induction in sediment extracts.

Main Methods:

  • Sediments from Lake Erie were analyzed for cadmium (Cd), mercury (Hg), and lead (Pb) using ICP-AES.

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Last Updated: Jul 6, 2026

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Published on: May 26, 2023

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
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An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

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  • HepG2 cells were exposed to sediment extracts and specific metals (Cd, Pb, or combinations).
  • Metallothionein (MT) levels were measured using the cadmium-hemoglobin affinity assay.

    • Main Results:

    • A strong correlation was found between MT induction in HepG2 cells and total metal/lead levels in sediments.
    • Cadmium was identified as the primary inducer of MT, even in the presence of lead.
    • MT levels increased significantly and in a concentration-dependent manner with cadmium exposure.

    Conclusions:

    • The HepG2 cell line serves as a viable bioassay for assessing metal contamination in aquatic sediments.
    • Metallothionein (MT) induction in HepG2 cells is a reliable biomarker for monitoring metal exposure.
    • This bioassay system can help monitor complex metal mixtures in environmental samples.