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Updated: Jul 6, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Quantitative real-time PCR is not more sensitive than "conventional" PCR

Patrick Bastien1, Gary W Procop, Udo Reischl

  • 1University of Montpellier 1/Centre Hospitalier Universitaire de Montpellier, Laboratory of Parasitology-Mycology, Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Montpellier, France. p-bastien@chu-montpellier.fr

Journal of Clinical Microbiology
|April 11, 2008
PubMed
Summary

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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